Entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) into host cells depends on refolding of the virus-encoded spike protein...
CryoEM
Sample preparationThe genes encoding ISDra2 TnpB (TnpB from D. radiodurans ISDra2; residues 1 to 408) and the 247-nt ωRNA were synthesized by...
Inflammasomes are cytosolic innate immune complexes that activate caspase-1 upon detection of pathogenic and endogenous dangers1-5, and NLRP3 is an...
Yeast strainsYeast strains were constructed using classical recombination-based techniques. The ∆Sea1CS strain was generated using CRISPR-based mutagenesis. Strains used in...
Previously, it has been impossible to capture the high-resolution images of nitrogenase, the only enzyme capable of reducing nitrogen into...
Synthetic nucleotide ligandsSynthetic CDN ligands were purchased from Biolog Life Science Institute: c-di-GMP (catalogue number C 057) and 3′,3′-cGAMP (catalogue...
Bacterial strains and constructsStrains, plasmids, constructs and oligonucleotides used in this study are shown in Supplementary Table 3.Expression and purification...
Expression and purification of human USP14Wild-type USP14 and its mutants were cloned into pGEX-4T vector obtained from GenScript (Nanjing, China)....
When something goes wrong during DNA replication, cells call their own version of 911 to pause the process and fix...
Author notesYukihiko SugitaPresent address: Laboratory of Advanced Protein Characterization, Research Center for State-of-the-Art Functional Protein Analysis, Institute for Protein Research,...