Hampoelz, B., Andres-Pons, A., Kastritis, P. & Beck, M. Structure and assembly of the nuclear pore complex. Annu. Rev. Biophys....
pore
Constructs and mutagenesisThe coding sequences of full-length GSDMs were cloned into a pET28-His-SUMO vector after the N-terminal His6-SUMO tag. In...
Cell cultureWild-type HeLa Kyoto cells (RRID: CVCL_1922) were a gift from S. Narumiya; their genome was sequenced previously39. Cells were...
U.S. Energy Information Administration. International Energy Outlook 2021 (Narrative) (U.S. Energy Information Administration, 2021).Rufford, T. E. et al. The removal...
Research from the lab of Peng Bai, assistant professor of energy, environmental and chemical engineering at the McKelvey School of...
Nuclear pore complexes (NPCs) are massive multi-protein complexes that act as passageways for the transport of molecules into and out...
Cell culture and Nup96 depletionNUP96::Neon-AID DLD-1 cells23 were grown in standard tissue culture conditions (37 °C with 5% CO2) using DMEM...
Context matters. It's true for many facets of life, including the tiny molecular machines that perform vital functions inside our...