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Chip fabrication and testingOur experimental results were measured on chips built from 300-mm wafers with a 14-nm complementary metal-oxide-semiconductor front...
transcription
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Expression, purification and reconstitution of human octamer histonesHuman histones were expressed and purified as described previously57. Lyophilized histones were mixed...
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Protein expression, mutagenesis and purificationXenopus laevis histones for nucleosome assembly were overexpressed in the Escherichia coli BL21(DE3) pLysS strain and...
Steroid hormone receptors are ligand-binding transcription factors essential for mammalian physiology. The androgen receptor (AR) binds androgens mediating gene expression...
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Nature, Published online: 25 January 2023; doi:10.1038/d41586-023-00121-1High-resolution structures of the bacterial Rho protein in complex with an RNA polymerase enzyme...
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Ray-Soni, A., Bellecourt, M. & Landick, R. Mechanisms of bacterial transcription termination. Annu. Rev. Biochem. 85, 319–347 (2016).Article CAS Google...
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Ray-Soni, A., Bellecourt, M. J. & Landick, R. Mechanisms of bacterial transcription termination: all good things must end. Annu. Rev....
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DNA methylation is a major epigenetic modification that is crucial for mammalian development. For instance, DNA methylation is central to...
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Culture of embryonic stem cellsAll cell lines are based on E14 mES cells, provided by E. Heard’s laboratory. Cells were...
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CU Boulder researchers have discovered a new way to inhibit the most commonly mutated gene underlying human tumor growth, opening...