a,b, DHODH, GPX4 and Vinculin protein levels in different cell lines were determined by western blotting. c–i, Chemical structures of iFSP1 (c), BQR (d), BAY-2402234 (e), AG-636 (f), DSM265 (g), PTC299 (h) and Vidofludimus (i). j–m, Cell viability measurement in HT-1080 cells treated with RSL3, AG-636 (100 μM) (j), DSM265 (100 μM) (k), PTC299 (100 μM) (l) and Vidofludimus (100 μM) (m) for 24 h. n,o, FSP1 and Vinculin protein levels in different cell lines were determined by western blotting. p, Cell viability measurement in Vector and FSP1 overexpression Hs 578T cells treated with RSL3 for 24 h. q, Cell viability measurement in HT-1080 cells treated with RSL3, BQR (1 μM) and Lip-1 (1 μM) for 24 h. r, Cell viability measurement in HT-1080 cells treated with RSL3, BAY-2402234 (1 μM) and Lip-1 (1 μM) for 24 h. In a,b,j–r, cells were grown in uridine (50 μM) supplemented medium. Data are presented as mean values +/− SD, n = 3 (j–m,p–r). Data are from three independent replicates (j–m,p–r). Western blot is representative of two independent replicates (a,b,n,o).
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